The synergistic action of gold nanoparticles (AuNPs) as hypoallergenic carriers and Gentiana lutea root extract (GRE) with healing, anti-inflammatory, and antibacterial properties can favourably affect skin re-epithelialization by stimulating the synthesis of proteins and lipids involved in the epidermal barrier formation. Our previous research has shown that NPs of a certain size and concentration in combination with herbal components show a better effect due to synergistic interaction compared to those applied alone, which is reflected through the cytotoxicity reduction. AuNPs synthesized by laser ablation in the presence of GRE will be tested in vitro on keratinocyte and fibroblast cell lines to determine the optimal concentration that activates cell proliferation. In order to create a functional nanocomposite material that offers a novel approach to the therapy of damaged skin and can be considered safe for long-term and frequent use, the optimal concentration of colloid GREAuNPs suspension will be combined with a layer of biocompatible, biodegradable, and physico-chemically inert bacterial cellulose.